Table 1.

Summary of in vitro and in vivo phenotypes of Sae2 mutants

	DNA binding	MRX-DNA binding	ssDNA endonuclease activity	hairpin endonuclease activity	sporulation (% of wt)	LYS2 Conversion (median frequency ± SD × 107)
Sae2 wt	+	+	+++	+++	81.4 ± 9	1950 ± 440
Sae2(G270D)	−	−	−/+	−	0.2 ± 0.4	23 ± 2
Sae2(5D)	+	+	+++	+++	0.2 ± 0.4	170 ± 160
Sae2(5A)	+	+	++	−	< 0.2	40 ± 21
Sae2(ΔN)	−	−	−	−	< 0.2	31 ± 17
Sae2(ΔC)	+	+	++	−	< 0.2	21 ± 18
vector only					< 0.2	20 ± 5.5