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. 2007 Oct 18;149(1):122–130. doi: 10.1210/en.2007-0705

Figure 2.

Figure 2

Electron microscopic visualization evidence for swim stress-induced internalization of CRFr in LC dendrites. Sections from a control rat (A) and a rat perfused 1 h (B) and a rat perfused 24 h (C) after 15-min swim stress. A, Immunogold-silver labeling for CRFr (arrowheads) can be seen along the plasmalemma in a dendritic profile. The dendrite receives synaptic contacts from axon terminals (t). B and C, CRFr labeling shifts from the plasmalemma to the cytoplasm 1 h (B) and 24 h (C) after 15-min swim stress, respectively. Arrows point to immunogold-silver labeling in the cytoplasm. Double-headed arrows point to endosome-like vesicles. Asterisks indicate astrocytic processes. D and E, Electron photomicrographs showing TH-immunoperoxidase-labeled dendrites (TH-d) containing immunogold-silver labeling for CRFr in control (D) and 24 h (E) after 15-min swim stress. CRFr labeling shifts from the plasmalemma to the cytoplasm in TH-immunoperoxidase-labeled dendrites 24 h after 15-min swim stress. Arrows point to immunogold-silver labeling in the cytoplasm, whereas arrowheads point to immunogold-silver labeling on the plasma membrane. dcv, Dense-core vesicles; m, mitochondria. Scale bar, 0.5 μm.