Figure 4.

Electron microscopic evidence for stress-induced CRFr internalization in LC perikarya containing immunogold-silver labeling for CRFr and immoperoxidase labeling for TH. A, Electron photomicrograph showing immunogold-silver labeling for CRFr (arrowheads) along the plasma membrane in control rats; B, Electron photomicrograph from a rat perfused 24 h after 15-min swim stress. CRFr labeling shifts from the plasma membrane to the cytoplasm 24 h after 15-min swim stress in TH-labeled perikarya. Arrows point to immunogold-silver labeling in the cytoplasm, whereas arrowheads point to immunogold-silver labeling on the plasma membrane. Nc, Nucleolus; N, nucleus. Scale bar, 0.5 μm.