Figure 7.

Attenuation of CRFr internalization by antagonist pretreatment. A, Electron photomicrograph showing immunogold-silver labeling for CRFr along the plasmalemma (arrowheads) in a dendrite of a control rat; B, CRFr labeling is more prominent in the cytoplasm in a dendrite from a subject pretreated with vehicle before swim and perfused 24 h after 15-min swim stress; C, A dendrite from an antagonist-pretreated subject exposed to swim stress and perfused 24 h later indicating a reduction in CRFr internalization. Arrows point to immunogold-silver particles distributed within the cytoplasmic compartment, whereas arrowheads point to immunogold-silver labeling on the plasma membrane. m, Mitochondria; t, terminal; ud, unlabeled dendrite. Scale bar, 0.5 μm.