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. 2007 Sep 20;149(1):329–338. doi: 10.1210/en.2007-0594

Figure 4.

Figure 4

Colocalization of Stat5a with PRA or PRB. A, Staining for Stat5a and PRA was carried out on sections from 3- and 10-d E+P-treated ovariectomized mice and was visualized with a Nikon Eclipse fluorescent microscope. Staining for Stat5a and PRB was carried out on sections from 10-d E+P-treated mice only and was visualized with a Pascal laser scanning confocal microscope. Yellow arrowheads indicate Stat5a and PRA or Stat5a and PRB colocalization (yellow nuclei). White arrowhead indicates Stat5a-negative, PRA-positive cell (red nucleus). Green arrowheads indicate Stat5a-positive, PRA- or PRB-negative cells (green nuclei). Scale bar, 20 μm. B, Quantitation of Stat5a and PR colocalization in 3- and 10-d E+P-treated animals. The values represent the mean ± sem from three animals per group, with a minimum of 800 cells per mouse analyzed. The percentage of PRA-positive cells and Stat5a/PRA-positive cells were both significantly lower in 10-d E+P alveolar structures than 3-d E+P ducts (P < 0.001) as determined by Student’s t test.