Table 3.
Microarray results of intestinal genes up-regulated, under fructose perfusion only, in 20 d relative to those in 10-d-old pups
| Access no. | Symbol | Name | Mean fold change 20 vs. 10F | se | Frequency |
|---|---|---|---|---|---|
| Metabolic activity | |||||
| U33500 | Rdh2 | Retinol dehydrogenase type 2 | 5.10 | 2.06 | 5 |
| NM_012608 | Mmed | Membrane metallo endopeptidase | 3.12 | 1.57 | 5 |
| NM_019168 | Arg2 | Arginase 2 | 3.05 | 1.32 | 5 |
| NM_012600 | Me1 | Malic enzyme 1 | 2.07 | 0.28 | 5 |
| NM_012569 | Gls | Glutaminase | 1.98 | 0.24 | 5 |
| NM_017156 | Cyp2b15 | Cytochrome P450, 2b15 | 1.81 | 0.57 | 4 |
| NM_017085 | Cyp19 | Cytochrome P450, subfamily 19 | 1.80 | 0.51 | 4 |
| AF121345 | Phyh | Phytanoyl-CoA hydroxylase (Refsum disease) | 1.78 | 0.20 | 4 |
| U03629 | Gda | Guanine deaminase | 1.66 | 0.17 | 5 |
| Transcription regulation/transcription factor | |||||
| NM_017115 | Myog | Myogenin | 2.31 | 0.16 | 4 |
| AB026288 | Cebpd | CCAAT/enhancer binding, protein (C/EBP) δ | 1.61 | 0.10 | 5 |
| Signal transduction activity | |||||
| M92920 | Phkb | Phosphorylase kinase, β | 5.61 | 2.26 | 5 |
| AJ130946 | Kpna2 | Karyopherin (importin) α-2 | 2.33 | 0.80 | 5 |
| U32434 | Rgs3 | Regulator of G protein signaling 3 | 1.84 | 0.52 | 5 |
| Receptor activity | |||||
| AF177685 | Pcdha11 | Protocadherin α 11 | 2.31 | 0.88 | 4 |
| NM_019295 | Cd5 | CD5 antigen | 2.18 | 0.71 | 4 |
| AF016184 | LOC286985 | Putative pheromone receptor (Go-VN7) | 1.61 | 0.35 | 4 |
| AF016178 | LOC286915 | Putative pheromone receptor (Go-VN1) | 1.60 | 0.23 | 4 |
| Hormone metabolism | |||||
| X91234 | Hsd17b2 | 17-β Hydroxysteroid dehydrogenase type 2 | 2.03 | 0.63 | 5 |
| U89280 | Hsd17b6 | 17-β Hydroxysteroid dehydrogenase type 6 | 1.77 | 0.71 | 4 |
| NM_017092 | Tyro3 | TYRO3 protein tyrosine kinase 3 | 1.67 | 0.51 | 5 |
| Unclassified | |||||
| M83209 | Psp | Parotid secretory protein | 4.47 | 2.98 | 5 |
| X60661 | Potential ligand-binding protein, RYD5 | 2.81 | 1.03 | 4 | |
| S82649 | Narp | Neuronal activity regulated pentraxin | 2.65 | 0.74 | 4 |
| AF078778 | Microtubule-associated protein 1B, noncoding exon 3U | 2.56 | 0.47 | 5 | |
| AF168362 | Pass1 | Protein associating with small stress protein PASS1 | 1.85 | 0.73 | 5 |
| AJ005161 | Mitochondrial translational elongation factor | 1.82 | 0.41 | 4 | |
| M93401 | Mmsdh | Methylmalonate semialdehyde dehydrogenase gene | 1.64 | 0.14 | 4 |
Up-regulated genes must have changed by more than 1.5-fold in at least four of five samples, and the fold change must be significant (P < 0.05). Values are means ± se for each gene (n = 5). Values indicate the fold increase in the expression of a gene in 20-d-old fructose-perfused compared with 10-d-old fructose-perfused intestine.