Table 5.
Microarray results of intestinal genes differentially regulated under glucose or fructose perfusion between 20- and 10-d-old pups
| Access no. | Symbol | Name and function | 20F vs. 10F
|
20G vs. 10G
|
||||
|---|---|---|---|---|---|---|---|---|
| Mean fold change | se | Freq | Mean fold change | se | Freq | |||
| Up-regulated genes | ||||||||
| Metabolism | ||||||||
| NM_013061 | Si | Sucrase isomaltase | 19.89 | 5.98 | 5 | 29.40 | 10.85 | 5 |
| NM_017013 | Gsta2 | Glutathione-S-transferase, α type2 | 7.45 | 4.67 | 5 | 10.12 | 6.86 | 5 |
| M25148 | Pla2g2a | Phospholipase A2, group IIA | 6.38 | 6.14 | 5 | 5.08 | 2.26 | 5 |
| D10354 | Gpt | Glutamic-pyruvate transaminase (alanine amino-transferase) | 4.99 | 1.72 | 5 | 5.33 | 1.37 | 5 |
| L81170 | Cyp2j4 | CYP2J4 | 4.52 | 2.97 | 5 | 7.64 | 4.43 | 5 |
| AF111160 | Gsta1 | Glutathione S-transferase, α-1 | 3.92 | 1.50 | 4 | 6.22 | 3.54 | 5 |
| Nucleotide/DNA metabolism | ||||||||
| NM_013097 | Dnase1 | Deoxyribonuclease I | 11.04 | 2.44 | 5 | 10.30 | 3.84 | 5 |
| U90888 | Ampd3 | Adenosine monophosphate deaminase 3 | 4.33 | 1.97 | 5 | 6.15 | 3.43 | 5 |
| Signal transduction activity | ||||||||
| AB002801 | Cnga3 | Cyclic nucleotide gated channel α 3 | 7.10 | 3.24 | 5 | 2.90 | 1.31 | 5 |
| U44750 | Hpgd | NAD-dependent 15-hydroxyprostaglandin dehydro-genase | 5.95 | 3.51 | 5 | 5.83 | 1.83 | 5 |
| Development/proliferation/differentiation | ||||||||
| AF205717 | LRTM4 | Tetraspan protein | 3.90 | 1.59 | 5 | 7.57 | 2.88 | 5 |
| NM_017037 | Pmp22 | Peripheral myelin protein 22 | 2.17 | 0.89 | 5 | 6.27 | 2.85 | 5 |
| Transport | ||||||||
| D13871 | Slc2a5 | Fructose transporter | 7.12 | 6.20 | 4 | 2.70 | 0.93 | 4 |
| AF058714 | SDCT1 | Sodium-dicarboxylate cotransporter | 2.38 | 1.29 | 4 | 5.36 | 2.93 | 5 |
| Immunity/inflammation | ||||||||
| U16683 | Defensin RatNP-3 precursor | 3.91 | 4.22 | 5 | 10.50 | 6.36 | 5 | |
| Unknown | ||||||||
| AF296690 | Rattus norvegicus unknown mRNA | 5.61 | 2.13 | 5 | 4.91 | 2.42 | 5 | |
| Z30584 | Zg-16p | ZG-16p protein | 5.35 | 3.37 | 5 | 5.27 | 1.12 | 5 |
| Down-regulated genes | ||||||||
| Metabolism | ||||||||
| D00680 | Gpx3 | Glutathione peroxidase 3 | −14.29 | 2.36 | 5 | −16.67 | 4.59 | 5 |
| AF154349 | Prsc1 | Protease, cysteine, 1 (legumain) | −10.00 | 3.21 | 5 | −10.00 | 3.78 | 5 |
| J02773 | Fabp3 | Fatty acid binding protein 3 | −5.88 | 2.25 | 5 | −6.25 | 3.25 | 4 |
| AB048711 | Dpp7 | Dipeptidylpeptidase 7 | −5.00 | 2.03 | 5 | −7.69 | 3.01 | 5 |
| NM_012562 | Fuca | Fucosidase, α-l-1, tissue | −4.76 | 1.89 | 5 | −16.67 | 5.30 | 5 |
| NM_013156 | Ctsl | Cathepsin L | −4.17 | 1.90 | 5 | −16.67 | 4.78 | 5 |
| NM_012939 | Ctsh | Cathepsin H | −3.57 | 2.01 | 4 | −5.56 | 2.12 | 5 |
| J00705 | Apoe | Apolipoprotein E | −3.03 | 1.20 | 5 | −5.56 | 3.01 | 4 |
| M33648 | Hmgcs2 | 3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 | −2.78 | 0.98 | 4 | −5.88 | 3.20 | 4 |
| NM_012558 | Fbp1 | Fructose-1,6- biphosphatase 1 | −2.70 | 0.68 | 5 | −5.26 | 2.31 | 5 |
| Signal transduction | ||||||||
| D10233 | Renbp | Renin-binding protein | −6.25 | 1.96 | 5 | −7.14 | 2.31 | 5 |
| U24489 | Tnxa | Tenascin XA | −5.26 | 2.11 | 5 | −11.11 | 3.21 | 5 |
| AB016536 | Hnrpab | Heterogeneous nuclear ribonucleoprotein A/B | −3.45 | 1.20 | 5 | −9.09 | 3.54 | 4 |
| Development/proliferation/differentiation | ||||||||
| NM_012493 | Afp | α -Fetoprotein | −9.09 | 2.01 | 5 | −25.00 | 6.54 | 5 |
| AF045657 | Dab2 | Disabled homolog 2, mitogen-responsive phosphoprotein | −4.55 | 1.98 | 5 | −7.69 | 2.02 | 5 |
| Transport | ||||||||
| L37380 | LOC252882 | Apical early endosomal glycoprotein | −8.33 | 2.15 | 5 | −16.67 | 3.58 | 5 |
| AF219904 | Folr1 | Folate receptor 1 (adult) | −6.25 | 2.23 | 5 | −11.11 | 2.89 | 5 |
| AB003400 | Dao1 | d-amino acid oxidase | −4.55 | 1.45 | 5 | −7.69 | 3.78 | 5 |
Genes up- and down-regulated that changed by more than 5-fold in four of five samples in at least one sugar perfusion condition (fructose or glucose perfusion) and whose the fold change is significant (P < 0.05). Values are means ± se for each gene (n = 5 experiments). Values in the ″20F vs. 10F″ column indicate the fold change in the expression of a gene in 20-d-old compared with 10-d-old fructose-perfused intestine. Values in the ″20G vs. 10G″ column indicate the fold change in the expression of a gene in 20-d-old compared with the 10-d-old glucose-perfused intestine. Freq, Frequency.