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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Gastroenterology. 2007 Oct 17;134(1):120–130. doi: 10.1053/j.gastro.2007.10.023

Figure 1. Growth characteristics of IEC-6 cells containing inducible KRASV12G.

Figure 1

(A) IEC-iKRAS cells were treated with or without 5 mM IPTG for up to three days. Cells were collected each day and cell lysates subjected to Western blot analyses using KLF5, KRAS and β-actin (loading control) antibodies. (B) Representative images of IEC-iKRAS cells with and without IPTG induction for 3 days. (C) Cell proliferation assay performed on cells treated or not with IPTG. N = 6; * p < 0.05 by two-tailed t test. (D) Anchorage-independent assay was conducted in soft agar in cells treated or not with IPTG for 21 days. N = 6; * p < 0.01.