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. Author manuscript; available in PMC: 2008 Dec 21.
Published in final edited form as: Immunity. 2007 Dec 21;27(6):952–964. doi: 10.1016/j.immuni.2007.11.014

Figure 1. Macrophages produce IL-10 in response to apoptotic cells.

Figure 1

(a) Apoptosis of Jurkat cells was induced 0, 3, 6, and 12 hrs. The percentage of early and late-apoptotic cells was quantified by flow cytometry analysis using Annexin V and propidium iodide (PI) staining. The numbers indicate percentages of the subpopulations.

(b-d) 0.5 × 105 thioglycollate-elicited peritoneal macrophages (c and e) or HMDM (b), or RAW264.7 cells (d) were stimulated with LPS (0.5μg/ml), apoptotic Jurkat cells, autologous apoptotic CD4+ T cells (ac), live cells (lc), or necrotic cells (nc), (2:1 ratio of ac, lc or nc/macrophages) for indicated times, and analyzed for IL-10 production.

(e) BALB/c mice (3 per group) were injected i.v. with apoptotic or necrotic Jurkat cells at doses of 2×106 and 106 cells/mouse. Blood were collected and sera obtained 4 and 8 hr following the injections. Serum IL-10 levels were measured by ELISA.

JKT ac, apoptotic Jurkat T cells (used in b-e); autologous ac, apoptotic splenic CD4+ T cell (b) or purified human blood CD4+ T cells (c). All data are presented as mean ± SD from a minimum of three individual donors.