Skip to main content
. 2003 Oct 15;31(20):5957–5970. doi: 10.1093/nar/gkg788

Figure 1.

Figure 1

Activity of FETUAcat constructs and TF abundance in human hepatoma cells. (A) The basal activity of p-3320/48cat and p-273/48cat in NCM as well as their responsiveness to CM was tested by transient transfection in HepG2 or Hep3B cells. Every Cat activity is relative to the activity (100%) of the pSV2cat plasmid (hatched bar) transfected in NCM-treated cells. For every cat construct, the CM-induced activation or repression relative to the reference activity in NCM-cultured cells is indicated by a broken arrow and a value (folds). (B) Amounts of NF-1 and C/EBP family members in nuclear extracts from Hep3B or HepG2 cells or rat liver. Total nuclear proteins (15 µg/lane) were separated by SDS–PAGE, electro-transferred and immunodetected with the antisera noted on the left. Each protein band is identified on the right with a p followed with the size (kDa) of an expected NF-1 or C/EBP isoform (35–37). A Coomassie blue staining of total proteins before electro-transfer is shown in the lower panel. At the bottom is the anode.