Figure 6.

Phasing of NF-1 and C/EBP boxes in the FETUA promoter and CM-induced responsiveness. Two mutants of p-200/48cat lacking either 5 Ts (pΔ5-200/48cat) or 10 nt, namely dGdT₉ (pΔ10-200/48cat) disrupt or retain the phasing between (N1+C1) and (N2+C2), as depicted. These mutants were co-transfected with pC/EBPα or pHD. Promoter activities are relative to p-200/48cat activity (100%) in the presence of pHD and NCM. For every cat construct the CM-induced repression relative to the reference activity in NCM-cultured cells is indicated by a broken arrow and a value (folds). Other details are as in Figure 3.