Table 1.
Previous studies employing microarray approaches to study stroke
| Soriano et al. 2000 | Jin et al. 2001 | Kim et al. 2002 | Rao et al. 2002 | Schmidt-Kastner et al. 2002 | Tang et al. 2002 | Roth et al. 2003 | Kim et al. 2004 | Lu et al. 2004 | Moore et al. 2005 | Ford et al. 2006 | Tang et al. 2006 | Vikman and Edvinsson 2006 | Our data | |
| Material used | Rat brain tissue | Rat brain tissue | Rat brain tissue | Rat brain tissue | Rat brain tissue | Rat brain tissue | Rat brain tissue | Rat brain tissue | Rat brain tissue | Rat brain tissue | ||||
| Model of ischemia | Permanent focal MCAO | Transient global MCAO | Permanent focal MCAO | Transient focal MCAO | Transient focal MCAO | Permanent focal MCAO | Permanent focal MCAO | Transient focal MCAO | Transient focal MCAO | Blood from ischemic stroke patients | Permanent and transient focal MCAO | Blood from ischemic stroke patients | Post-mortem brain tissue from 11 stroke patients | Post-mortem brain tissue from 12 stroke patients and permanent focal rat MCAO |
| No of genes | 750 | 374 | 1176 | 1263 | 9044 | ~8,000 | ~13,000 | 5,000 | 1,322 | ~19,000 | 8784 | ~39,000 | 7458 | 1176 |
| Time after ischemia | 3 hours | 4 hours 24 hours 72 hours |
6 hours | 6 hours 24 hours |
5 hours | 24 hours | 1 hours 3 hours 6 hours 24 hours |
3 hours 6 hours 12 hours 1 days 2 days 4 days |
30 min 4 hours 8 hours 24 hours 3 days 7 days |
As soon as possible after hospitalization | 24 hours | 3 hours 5 hours 24 hours |
7–10 days (obtained 2–3 days post-mortem) | 1 hour-21 days (rat) and 2–37 days (human, obtained by 6 hours post-mortem) |
| Cut-off values | 2.0-fold | 1.7-fold | 2.0-fold | 2.5-fold | 1.7-fold | 2.0-fold | 3.0-fold | 2.0-fold | 2.0-fold | - | 2.0-fold | 1.5-fold | - | 2.0-fold |
| Confirmation of results | In situ hybridization, western blotting | Western blotting, immuno-histochemistry | RT-PCR | Real-time PCR, antisense knockdown, western blotting, immuno-histochemistry | Microarray analysis only | Real-time RT-PCR | Cell culture, in situ hybridization, western blotting, immuno-fluorescence | Cell culture, northern blotting, RT-PCR, western blotting, immuno-histochemistry | Real-time RT-PCR | Real-time RT-PCR | Microarray analysis only | Microarray analysis only | Real-time PCR, immuno-histochemistry | Cell culture, RT-PCR, western blotting, immuno-histochemistry, immuno-fluorescence |
| Selected molecules | NGFI-C ARC |
GRB2 SMN1 |
IFN-IP NDGAP-1 NPR |
SOCS-3 | NARP SPR SPIN2C ARG1 LBP |
PC4 | FAK | Synaptic proteins | CD14 CD36 FcGR2A IFNGR1 caspase-1 a-catenin |
LY64 ELK3 POU3F4 RHOA |
PAK1 MMP11 INI1 |
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