Table 3.
Sites under positive selection on the primate ANG gene
| Sites | PAML ω/PP (BEB) | TreeSAAP properties | ||||||||
| Codo n | Amino acid | M2a | M8 | Total | Chemical | Structural | Other | |||
| 28 | 4 Ser | 3.376 ± 0.53 0.999 | 3.210 ± 0.518 1.000 | 9 | 4 | RF, Hnc, Esm,Ra | 5 | F, Pc,BI, α n, P | 0 | ---- |
| 31 | 7 Thr | 3.246 ± 0.735 0.946 | 3.158 ± 0.610 0.980 | 4 | 3 | RF, pK', H | 1 | αn | 0 | ---- |
| 32 | 8 His | 3.245 ± 0.73 0.943 | 3.166 ± 0.590 0.982 | 1 | 0 | --------- | 1 | αc | 0 | ---- |
| 35 | 11 Thr | 3.369 ± 0.547 0.996 | 3.208 ± 0.521 0.999 | 3 | 0 | --------- | 3 | Pβ, K0, αc | 0 | ---- |
| 56 | 32 Arg | 3.246 ± 0.73 0.943 | 3.157 ± 0.610 0.979 | 5 | 2 | pHi, p | 3 | F, αc, P | 0 | ---- |
| 58 | 34 Gly | 3.229 ± 0.740 0.936 | 3.160 ± 0.607 0.979 | 9 | 7 | Br, RF, El, Hnc, Ra, Hp, Et | 2 | Ns, Pβ | 0 | ---- |
| 65 | 41 Asp | 3.263 ± 0.709 0.950 | 3.173±0.587 0.984 | 7 | 3 | pK', Pr, p | 3 | K0, F, α c | 1 | Mw |
| 75 | 51 Arg | 3.349 ± 0.584 0.986 | 3.202 ± 0.534 0.996 | 5 | 2 | pHi, Hnc | 3 | K0,αc, P | 0 | ---- |
| 76 | 52 Ser* | 3.247 ± 0.729 0.946 | 3.166 ± 0.598 0.983 | 0 | 0 | --------- | 0 | -------- | 0 | ---- |
| 87 | 63 Asn | 3.335±0.607 0.980 | 3.199 ± 0.540 0.995 | 9 | 4 | Br, μ, Pr, p | 4 | Ns, α n, BI, V0 | 1 | Mw |
| 90 | 66 Arg | 3.318 ± 0.635 0.974 | 3.191 ± 0.555 0.992 | 16 | 9 | Br, RF, pK', pHi, EI, Ra, Hp, Ht, Et | 7 | F, Mv, α c,Ns, P β, BI, P | 0 | ---- |
| 106 | 82 Lys | 3.287 ± 0.680 0.960 | 3.178 ± 0.579 0.987 | 4 | 3 | Br, pHi, Et | 1 | Ns | 0 | ---- |
| 108 | 84 His | 3.368 ± 0.550 0.995 | 3.207 ± 0.524 0.998 | 6 | 3 | pHi, Hnc,p | 3 | F, α c, P | 0 | ---- |
| 117 | 93 Gln | 3.367 ± 0.551 0.995 | 3.208 ± 0.523 0.999 | 5 | 3 | Br, pHi, Et | 2 | BI, αc | 0 | ---- |
| 127 | 103Val | 3.229 ± 0.75 0.936 | 3.150 ± 0.628 0.976 | 1 | 0 | --------- | 1 | αn | 0 | ---- |
ω and Bayesian (BEB) analysis posterior probabilities obtained with the NJ topology are shown for sites with PP > 0.95 in M8 that also have a PP > 0.90 in M2a. TreeSAAP analysis results present the total number of radical changes in amino acid properties and their assigned categories. Type I sites are shown in bold. *Site 52 had only strong support when using the NJ topology.
Properties symbols are as following: α c: Power to be – C-term., α-helix; α n: Power to be in the N-terminal of an α-helix; Br: Buriedness; BI: Bulkiness; EI: Long-range non-bonded energy; Esm: Short and medium range non-bonded energy; Et: Total non-bonding Energy; F: Mean r.m.s. fluctuation displacement; H: Hydropathy; Hnc: Normal consensus hydrophobicity; Hp: Surrounding hydrophobicity; Ht: Thermodynamic transfer hydrophobicity; K0: Compressibility; μ: Refractive index; Mv: Molecular volume; Mw: Molecular weight; Ns: Average number of surrounding residues; Pα: α- helical tendencies; Pβ: β-structure tendencies; Pc: Coil tendencies; P: Turn tendencies; p: Polarity; pHi: Isoelectric point; pK': Equilibrium Constant of ionization for COOH; Pr: Polar requirement; Ra: Solvent accessible reduction ratio; RF: Chromatographic index; V0:Partial specific volume;