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Compound effects on RTA fluorescence and circular dichroism. (A) Fluorescence emission (circles) and far-UV CD (triangles) changes on titration with urea. (B) Fluorescence spectra in PBS buffer alone (solid line), 1 M urea (dashed line) and 2 mM adenine (dotted line). (C) Fluorescence in the presence of (a) 2 M formamide, (b) 1 M N-methylurea, (c) 1 M N, N-dimethylurea, (d) buffer alone, and (e) 1 M hydroxyurea, in order of decreasing maximal intensity.
