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Binding isotherms. (A) Fluorescence emission increase on titration of RTA with urea at 5°C (open circles) and 30°C (open triangles), and NMU at 5°C (filled circles) and 30°C (filled triangles). (B) Titration with adenine (diamonds) and formamide (squares) at 25°C. The axis for adenine is in millimolar (top). A Scatchard plot for adenine binding is inset.
