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. 2003 Oct 15;31(20):5817–5830. doi: 10.1093/nar/gkg801

Figure 2.

Figure 2

Figure 2

Binding of LNA ODNs to plasmid allows complementary DNA ODNs to bind. (A) Agarose gel (without EtBr) of 0.023 µM plasmid gWiz incubated with rhodamine-labelled LNA ODNs at high (H, 4 µM) or low (L, 0.5 µM) ODN concentrations. (B) As (A) with EtBr. Lane M, 1 kb DNA ladder. (C) Agarose gel (without EtBr) of 0.023 µM plasmid gWiz incubated with LNA ODNs at high (H, 4 µM) or low (L, 0.5 µM) ODN concentrations. Unbound LNA ODN was removed, the plasmid–LNA ODN complex was incubated with the fluorescein-labelled DNA primer KH2 (4 µM) and free ODN was again removed prior to loading on a gel (except lane 4). (D) As (C) with EtBr. Lane 1, gWiz + LNA6 H + KH2; lane 2, gWiz + LNA6 L + KH2; lane 3, empty; lane 4, gWiz + KH2.