Figure 3.

Neighbor-Joining phylogenic tree of non-ECF σ ⁷⁰ factors. The σ⁷⁰ sequences were from [13] or were retrieved from sequenced Burkholderia genomes or the GenBank database. Within a single species only one ortholog per sigma lineage was kept. A total of 178 sites were analyzed (with global removal of gap-containing sites). Distances are proportional to evolutionary divergences expressed in substitutions per 100 sites. Bootstrap values higher than 85% are given. Sub-groups are defined in the text. (*) B. xenovorans LB400 alternative primary factor. (**) additional copy of rpoD found in B. xenovorans LB400 genome. (***) Deduced B. dolosa AU0158 RpoD sequence that probably contains a frameshift due to a DNA sequencing mistake. Main phylogenetic groups of non-ECF factors are indicated on the figure: group 1, primary and alternative primary factors; group 2, stationary phase factors; and group 3, heat shock (RpoH), flagellin (FliA), and cellular differentiation factors