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. 2007 Sep 4;8:308. doi: 10.1186/1471-2164-8-308

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Copyright © 2007 Menard et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The ecfA (rpoE) gene duplication. (a) PCR analysis of the duplicated 40 kb ecfA region among Bcc genomes. Strains used are described in Table 2. PCR primers are presented in Table 3. The DNA size marker is shown. Orientation of the PCR primers is indicated in the b and c sections. B/C primers detect the Bcen-J2315 configuration (duplication event)-ecfA2 allele, and A/B primers detect the Burkholderia sp. 383 configuration (no inversion). (b) and (c) Promer plot comparison of Burkholderia sp. 383 chromosome 1 against either (b) Bcen-J2315 chromosome 1 or (c) the partially inverted Bcen-J2315 chromosome 1. inverted region #1 an inverted region #2 of Bcen-J2315. ISBcen8 position is indicated. Promer analyses were performed according to [25].

Inline graphic — The ecfA (rpoE) gene duplication. (a) PCR analysis of the duplicated 40 kb ecfA region among Bcc genomes. Strains used are described in Table 2. PCR primers are presented in Table 3. The DNA size marker is shown. Orientation of the PCR primers is indicated in the b and c sections. B/C primers detect the Bcen-J2315 configuration (duplication event)-ecfA2 allele, and A/B primers detect the Burkholderia sp. 383 configuration (no inversion). (b) and (c) Promer plot comparison of Burkholderia sp. 383 chromosome 1 against either (b) Bcen-J2315 chromosome 1 or (c) the partially inverted Bcen-J2315 chromosome 1. inverted region #1 an inverted region #2 of Bcen-J2315. ISBcen8 position is indicated. Promer analyses were performed according to [25].