Table 3.

PCR primers used in this study.

DNA targets	PCR primer code	DNA sequence	position on Bcen-J2315 genome	annealing temperature
ecfA1 region	A	CACGGTCATCACGCTCGC	Chr1 3124945-3124962	55°C
ecfA1 and ecfA2 region	B	TTCGGTGCGATAACCAGC	Chr1 1056358-1056374	55°C
ecfA2 region	C	CGGGCGCAAGAACATACA	Chr1 1056771-1056789	55°C
ecfB	ecfB-F	CGCTTCGCGCTCTGGCTG	Chr1 3817718-3817735	58°C
ecfB	ecfB-R	TCAGCCGCAAGGAAGGAGC	Chr1 3818171-3818153	58°C
ecfD	ecfD-F	CGCAACTGCGGCGCCCG	Chr1 2115044-2115060	55°C
ecfD	ecfD-R	CGAGCGTTTCGAGCGGGTC	Chr1 2615449-2615467	55°C
ecfE	ecfE-F	GTGAACGAACGAGGCTAGG	Chr1 2728191-2728210	55°C
ecfE	ecfE-R	CTCCTTGAAGGACTGTCC	Chr1 2727807-2727824	55°C
sigI	sigI-F	ATCCCGCTCGATGCCGCGC	Chr2 2667864-2667882	55°C
sigI	sigI-R	GAGAATTTGAAGCCGCGCCG	Chr2 2668242-2668261	55°C
sigI	sigIv-F	ATCATGCCCGCTGGCTC	Chr3* 1181202-1181218	55°C
sigI	sigIv-R	AACAGCCCGATGTTGCCT	Chr3* 1181508-1181491	55°C
sigJ	sigJ-F	CATGCGCATGGCACCCGTTC	Chr2 1383013-1383032	55°C
sigJ	sigJ-R	GAGAATTTGAAGCCGCGCCG	Chr2 2668242-2668261	55°C

* Position on B. vietnamiensis G4 genome