Figure 3.

The MRs of ARF5, ARF6, ARF7, and ARF8 contain ADs. (A) Transfections with truncated ARFs containing MRs plus the CTD. Effector plasmids consisted of the GAL4 DBD fused to the VP16 acidic AD from herpes virus or to ARF proteins that had their DBDs truncated. (B) Transfections with truncated ARFs containing only CTDs. Effector plasmids consisted of the GAL4 DBD fused to ARF proteins that had their DBDs plus MRs truncated. The reporter gene used in both A and B was the GAL4(4X) promoter-GUS gene (12). Effector genes contained the same promoter as those effectors tested in Fig. 2. The amino acid position of the amino termini in the truncated ARFs is indicated by N.