Figure 2.

Levels of c-FMS-specific mRNA increase significantly with monocyte/macrophage differentiation. Real-time PCR quantification of c-FMS mRNA levels relative to GAPDH in different human and mouse cell lines and cells from human donors and normal mice. The numbers indicate average relative mRNA levels from at least two independent RNA preparations. Fibroblasts, HeLa and 3T3 cells are c-FMS non-expressing cells. HL-60 cells express very low but detectable levels of c-FMS mRNA but PMA-induced monocyte/macrophage differentiation increases expression over 300-fold. Highest expression is observed in both normal human and mouse monocyte/macrophage cells and RAW 264 cells, a mouse macrophage cell line. ND, not done.