Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The MN digestion sensitivity of exons 1 and 2 of the DHFR gene in AT3-2, C26 and C38 cells. Nuclei were isolated from the three CHO cell lines and digested with MN (1 U/100 µl) for different times (0, 1, 3, 5 and 10 min). Genomic DNA was isolated, separated by electrophoresis in a 1.5% agarose gel, stained with ethidium bromide (A) and then transferred to a nitrocellulose membrane and hybridized with 32P-labeled probes specific for exon 1 (B) or exon 2 (C) of the DHFR gene.
