Figure 4.

KMnO₄ reactivity of the bottom (A) and top (C) strands of γ ori in the absence of Mg²⁺. pMF36 DNA template was incubated with His-tagged, copy-up π protein (250, 500 and 1000 ng) and/or ATP (4, 8 or 16 mM), as indicated by black, gray and dark gray triangles, respectively. All reactions were performed without Mg²⁺. For the control reaction, the reactivity of the probe to KMnO₄ in the absence of protein and ATP was examined. Quantification results for selected lanes are given (B and D). Bands of interest (singular or in clusters) are highlighted by small black arrows.