Figure 5.

Contributions of ATP and Mg²⁺ to the KMnO₄ signals within the γ ori. (A) The control lane (unlabeled) is a KMnO₄ reaction without ATP or Mg²⁺. Increasing concentrations of Mg²⁺ (0, 2.5, 5 and 10 mM) or ATP (0, 4, 8 and 16 mM) were used, as indicated by black and dark gray triangles, respectively. (B) Combinations of His-tagged, copy-up π protein (1 µg), Mg²⁺ (5 mM) and ATP (10 mM) were used to test the contributions of each of these components. Added components are indicated by ‘+’ and omitted components by ‘–’. Quantification results for (B) are shown in (C).