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. 2008 Jan 23;3(1):e1463. doi: 10.1371/journal.pone.0001463

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Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The egg cytosol was pre-incubated with EGTA, EDTA, EDTA with Mg²⁺ or EDTA with Ca²⁺ at optimum concentrations, and then mixed with E. coli suspension. After incubation at 25°C for 2 h, the bacteriolytic activities were measured by colony forming unit assay.