Figure 3.

Sequences of a pentaprobe and a triprobe. (A) One of the sequence solutions of a single double-stranded oligonucleotide (pentaprobe) that contains all 5 base elements exactly once. (B) The sequences of six overlapping double-stranded oligonucleotides (pentaprobe.1–pentaprobe.6) that together comprise a 5-complete solution. All possible 5 base sequences can be found exactly once in these oligonucleotides, with the exception of those sequences found in the overlap regions, which are represented twice. (C) The sequence (in bold) of a single double-stranded oligonucleotide (triprobe) that contains all 3 base elements exactly once. Flanking sequences to include BamHI and EcoRI restriction sites are shown in italic.