Figure 4.

Analysis of the DNA-binding activity of chosen GST fusion proteins. (A) Coomassie stained SDS–PAGE showing that each of the protein solutions used in subsequent EMSA experiments was similar in concentration. (B–G) EMSAs carried out using pentaprobe.1–pentaprobe.6 and the GST fusion proteins shown in (A). Lane 1, probe only; lane 2, GST; lane 3, GST fusion with GATA-N; lane 4, GST fusion with GATA-C; lane 5, GST fusion with BKLF; lane 6, GST fusion with Pegasus; lane 7, GST fusion with PU.1; lane 8, GST fusion with Eos; lane 9, GST fusion with FOG.