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Detection of artefacts from contaminating bacterial DNA-binding proteins using anti-GST serum. EMSA showing the DNA-binding activity of GST–GATA-C (lane 1). Lane 2 additionally contains anti-GST serum, while lane 3 contains preimmune serum. The supershifting of the retarded bands seen for lane 2 indicates that the GST fusion protein, rather than an irrelevant contaminant, is responsible for the retardation.
