Skip to main content
NCBI home page
The Journal of General Physiology logoLink to The Journal of General Physiology
. 1959 Jan 20;42(3):579–587. doi: 10.1085/jgp.42.3.579

IN VITRO STUDIES OF P32 UPTAKE IN MOUSE LIVER MITOCHONDRIA

Arthur B Edmunds Jr 1
PMCID: PMC2194924  PMID: 13620888

Abstract

Isolated mouse liver mitochondria were incubated in two types of P32-labelled sucrose-phosphate buffers. The first contained no added ATP or oxidizable substrate. The second contained added ATP. Samples were taken at specified times, up to 60 minutes, and analyses were made of the mitochondrial TCA-soluble inorganic P32 and the total mitochondrial residue P31 and P32. The results of the analyses showed that when the phosphorus inhibition index (the ratio of the amount of incubation inorganic phosphorus to the square of the amount of tyrosine in the mitochondria) was high, inorganic P32 uptake was low and vice versa. In accordance with established data, increased P32 uptake was obtained when ATP was added. ATP was found to stabilize the turnover of TCA-insoluble residue phosphorus as well as to maintain the TCA-soluble orthophosphate pool. These results support findings regarding the inhibitory and controlling effects of incubation medium phosphate in the regulation of inorganic phosphorus uptake.

Full Text

The Full Text of this article is available as a PDF (497.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALDRIDGE W. N. Liver and brain mitochondria. Biochem J. 1957 Nov;67(3):423–431. doi: 10.1042/bj0670423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARTLEY W., DAVIES R. E. Active transport of ions by sub-cellular particles. Biochem J. 1954 May;57(1):37–49. doi: 10.1042/bj0570037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BARTLEY W., DAVIES R. E., KREBS H. A. Active transport in animal tissues and subcellular particles. Proc R Soc Lond B Biol Sci. 1954 Mar 25;142(907):187–196. doi: 10.1098/rspb.1954.0020. [DOI] [PubMed] [Google Scholar]
  4. BRENNER-HOLZACH O., RAAFLAUB J. Die Korrelation zwischen der Schwellung isolierter Mitochondrien und dem Abbau der intramitochondrialen Adenosinnucleotide (ATP, ADP, AMP, CoA). Helv Physiol Pharmacol Acta. 1954;12(3):242–252. [PubMed] [Google Scholar]
  5. CRANE R. K., LIPMANN F. The relationship of mitochondrial phosphate to aerobic phosphate bond generation. J Biol Chem. 1953 Mar;201(1):245–246. [PubMed] [Google Scholar]
  6. FRIEDKIN M., LEHNINGER A. L. Esterification of inorganic phosphate coupled to electron transport between dihydrodiphosphopyridine nucleotide and oxygen. J Biol Chem. 1949 Apr;178(2):611–644. [PubMed] [Google Scholar]
  7. GREEN D. C., ATCHLEY W. A. Studies the cyclophorase system; incorporation of P32. Arch Biochem. 1949 Dec;24(2):359–374. [PubMed] [Google Scholar]
  8. HUNTER F. E., Jr, DAVIS J., CARLAT L. The stability of oxidative and phosphorylative systems in mitochondria under anaerobic conditions. Biochim Biophys Acta. 1956 Apr;20(1):237–242. doi: 10.1016/0006-3002(56)90282-7. [DOI] [PubMed] [Google Scholar]
  9. HUNTER F. E., Jr, FORD L. Inactivation of oxidative and phosphorylative systems in mitochondria by preincubation with phosphate and other ions. J Biol Chem. 1955 Sep;216(1):357–369. [PubMed] [Google Scholar]
  10. KIELLEY W. W., KIELLEY R. K. Myokinase and adenosinetriphosphatase in oxidative phosphorylation. J Biol Chem. 1951 Aug;191(2):485–500. [PubMed] [Google Scholar]
  11. MUDGE G. H., NEUBERG H. W., STANBURY S. W. The effect of 2,4-dinitrophenol on the formation of phosphoenolpyruvate by liver mitochondria. J Biol Chem. 1954 Oct;210(2):965–979. [PubMed] [Google Scholar]
  12. RAAFLAUB J. Uber den Wirkungsmechanismus von Adenosintriphosphat als Cofaktor isolierter Mitochondrien. Helv Physiol Pharmacol Acta. 1953;11(2):157–165. [PubMed] [Google Scholar]
  13. RAFTER G. W. A pyrophosphatase activity associated with mouse liver particles. J Biol Chem. 1958 Feb;230(2):643–648. [PubMed] [Google Scholar]
  14. RECKNAGEL R. O., MALAMED S. The osmotic nature of mitochondrial swelling produced by carbon tetrachloride and inorganic phosphate. J Biol Chem. 1958 Jun;232(2):705–713. [PubMed] [Google Scholar]
  15. SIEKVITZ P., POTTER V. R. Biochemical structure of mitonchodria. I. Intra-mitochondrial components and oxidative phosphorylation. J Biol Chem. 1955 Jul;215(1):221–235. [PubMed] [Google Scholar]
  16. STANBURY S. W., MUDGE G. H. Polyphasic response of respiration and aerobic phosphorylation to 2,4-dinitrophenol. J Biol Chem. 1954 Oct;210(2):949–964. [PubMed] [Google Scholar]
  17. STANBURY S. W., MUDGE G. H. Potassium metabolism of liver mitochondria. Proc Soc Exp Biol Med. 1953 Apr;82(4):675–681. doi: 10.3181/00379727-82-20216. [DOI] [PubMed] [Google Scholar]
  18. TAPLEY D. F. The effect of thyroxine and other substances on the swelling of isolated rat liver mitochondria. J Biol Chem. 1956 Sep;222(1):325–339. [PubMed] [Google Scholar]
  19. WALKENSTEIN S. S., WEINHOUSE S. Oxidation of aldehydes by mitochondria of rat tissues. J Biol Chem. 1953 Feb;200(2):515–523. [PubMed] [Google Scholar]
  20. WERKHEISER W. C., BARTLEY W. The study of steady-state concentrations of internal solutes of mitochondria by rapid centrifugal transfer to a fixation medium. Biochem J. 1957 May;66(1):79–91. doi: 10.1042/bj0660079. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of General Physiology are provided here courtesy of The Rockefeller University Press

RESOURCES