Figure 1.

Inhibition of binding of ¹²⁵I-LNP to chitin. Various concentrations of mono- and oligosaccharides were combined with 109 ng of ¹²⁵I-LNP and 250 μg of chitin in a total volume of 100 μl. B. japonicum USDA110 Nod factor (▪); R. sp. NGR234(NGR_A) Nod factor (▴); R. sp. NGR234(NGR_B) Nod factor (▿); R. meliloti Nod factor (●); N-acetylglucosamine (□); chitin disaccharide (▾); chitin tetrasaccharide (▵); chitin pentasaccharide (♦); chitin hexasaccharide (○). Most points represent single determinations. A sufficient amount of R. meliloti Nod factor was available only for the above assay; all other ligands showed similar results in assays with other chitin preparations.