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. 1999 May 11;96(10):5856–5861. doi: 10.1073/pnas.96.10.5856

Figure 3.

Figure 3

Effect of carbohydrate ligands on phosphatase activity of LNP isolated from D. biflorus roots. LNP (402 ng/ml) was preincubated for 1 hour in 10 mM MOPS buffer, pH 7.2, containing various concentrations of B. japonicum USDA110 Nod factor (▪), Rhizobium sp. NGR234(NGRA) Nod factor (▴), Rhizobium sp. NGR234(NGRB) Nod factor (▾), R. meliloti Nod factor (●), or cis-vaccenic acid (♦) and then assayed for phosphatase activity by using a final concentration of 3 mM Mg-ADP. Points are averages of duplicate determinations.