Table 1.

Effect of anti-LNP-serum on root hair deformation and nodulation of D. biflorus roots

Treatment	Root hairs deformed, %		Nodules on roots inoculated with rhizobia
	Inoculated	Uninoculated	Treated region of root	Region of root emerged after treatment
Untreated	69.5 ± 4.6	6.8 ± 1.3	4.0 ± 0.2	1.7 ± 0.2
Preimmunization serum	76.6 ± 7.4	3.2 ± 0.6	3.8 ± 0.5	2.4 ± 0.5
Anti-LNP-serum	17.2 ± 3.6	4.4 ± 0.7	0.3 ± 0.1	1.3 ± 0.3

The roots of three sets of 3-day-old D. biflorus plants were immersed for 1 hour in 1:100 dilutions of preimmunization serum, anti-LNP-serum or serum-free medium, washed, and transferred to growth pouches. Half of each set of plants was inoculated with Bradyrhizobium sp. 24A10. After 62 hours, the roots of six plants from each of the above sets were examined for root hair deformation. After 3 weeks, the number of nodules in the treated region as well as in the region of root that emerged after treatment were recorded. No nodules were observed on the roots that had not been inoculated with rhizobia. The nodulation data represent the results of two separate experiments, using 10 and 16 plants per set, respectively. Values given are averages ± SE.