Figure 1.

(A) LePT1 expression under P_i starvation. Northern blot analysis of total RNA (10 μg) isolated from the roots of tomato plants grown aeroponically and misted with a solution containing 250 μM P_i (R+) or no P_i (R−) for 5 days. (B) Accumulation of LePT1 protein in roots of tomato plants. Western blot of total proteins (20 μg per lane) isolated from the roots of tomato plants treated as described above. The antibodies isolated from eggs of immunized hens (Imm) react with a protein of ≈60 kDa, corresponding to the predicted size of LePT1 protein, in the roots of P_i-starved plants. This protein is not detected when antibodies from preimmune eggs (Pre imm) are used. M, molecular mass markers. (C) LePT1 protein accumulates rapidly in response to P_i starvation. Western blot of total proteins (20 μg per lane) isolated from roots of plants sprayed with 250 μM (+) or no (−) P_i for 12 or 24 hr. M, molecular mass markers.