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Phosphate-starvation-induced accumulation of LePT1 protein in roots can be reversed by resupplying Pi to the starved plants. Tomato plants were sprayed with nutrient solutions containing 250 μM (+) or no (−) Pi. After 5 days the Pi-starved plants were replenished with 250 μM Pi (R) or continued to grow in Pi-deficient medium (C) for 1, 3, or 5 days as indicated. Total proteins (20 μg per lane) isolated from roots were analyzed by Western blotting. M, molecular mass markers.
