Figure 3.

Induction of DBF2 and At-DBF2 by osmotic, salt, heat, and cold stresses, but not by oxidative stress. (a) Northern analysis (25) showing the kinetics of At-DBF2 induction in plants treated with 20% PEG 6000 and of DBF2 in yeast treated with 2 M sorbitol for the time indicated. (b) Northern analysis showing At-DBF2 expression in 10-day-old plants grown for 5 hr in control conditions (1), at 37°C (2), with 20% PEG 6000 (3), 1% NaCl (4), at 4°C (5), or with 0.4 mM H₂O₂ (6), and DBF2 expression in yeast cells grown for 1.5 hr in YPD (1), at 37°C (2), with 2 M sorbitol (3), with 1.2 M NaCl (4), at 4°C (5), or with 0.4 mM H₂O₂ (6). The time chosen (5 hr) corresponds to the highest induction seen in a. Control of loading has been done with ethidium bromide staining and is shown under each Northern analysis. (c) Western analysis of At-DBF2 in Arabidopsis. Samples are similar to those analyzed in b.