FIG. 2.

Detection of specific anti-MIC3 IgG antibodies in sera of CBA/J mice immunized with pMIC3i alone, by Western blotting using T. gondii antigen. Sera from individual mice were collected on day 14 and diluted 1:100. The reactivities of sera from immunized mice and from mice receiving empty plasmid pcDNA3 are shown. The band corresponding to the native MIC3 at 90 kDa is indicated by an arrow. The molecular masses are shown on the left; results from one of three similar experiments are shown. T+, positive control (anti-MIC3 MAb T4 2F3); T−, negative control (serum from a naive mouse).