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. 1999 Jul 1;190(1):135–140. doi: 10.1084/jem.190.1.135

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© 1999 The Rockefeller University Press

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Hypoxia–reoxygenation on angiotensin II–triggered relaxation and eicosanoid metabolism in BCA. (A) Effects of indomethacin, SQ 29548, CGS13080, SOD, L-NMMA, and L-NAME on angiotensin II–triggered relaxation (white bars) and the release of 6-keto-PGF_1α (black bars) and PGE₂ (hatched bars) in hypoxia–reoxygenated BCA. After having obtained a reference response to angiotensin II, the coronary strip was exposed to hypoxia for 40 min after 40-min reoxygenation in presence of indomethacin (10 μM), CGS13080 (10 μM), SQ-29548 (10 μM), PEG-SOD (500 U/ml), L-NMMA (10⁻⁴ M), or L-NAME (10⁻⁴ M). PGE₂ and 6-keto-PGF_1α in the media were analyzed by ELISA. Data represent means ± SEM from 10 experiments. (B) Effects of superoxide, NO, and concurrent administration of superoxide and NO on angiotensin II–induced vasorelaxation and prostaglandin release in BCA. After having obtained a reference response to angiotensin II, the coronary strip was exposed to superoxide generated from 10 mU/ml xanthine oxidase/100 μM hypoxanthine or to NO generated from 20 μM DEA-NO or superoxide plus NO. PGE₂ (hatched bars) and 6-keto-PGF_1α (black bars) in the media were analyzed by ELISA. Data represent means ± SEM from 12 experiments. White bars, relaxation.

Hypoxia–reoxygenation on angiotensin II–triggered relaxation and eicosanoid metabolism in BCA. (A) Effects of indomethacin, SQ 29548, CGS13080, SOD, L-NMMA, and L-NAME on angiotensin II–triggered relaxation (white bars) and the release of 6-keto-PGF_1α (black bars) and PGE₂ (hatched bars) in hypoxia–reoxygenated BCA. After having obtained a reference response to angiotensin II, the coronary strip was exposed to hypoxia for 40 min after 40-min reoxygenation in presence of indomethacin (10 μM), CGS13080 (10 μM), SQ-29548 (10 μM), PEG-SOD (500 U/ml), L-NMMA (10⁻⁴ M), or L-NAME (10⁻⁴ M). PGE₂ and 6-keto-PGF_1α in the media were analyzed by ELISA. Data represent means ± SEM from 10 experiments. (B) Effects of superoxide, NO, and concurrent administration of superoxide and NO on angiotensin II–induced vasorelaxation and prostaglandin release in BCA. After having obtained a reference response to angiotensin II, the coronary strip was exposed to superoxide generated from 10 mU/ml xanthine oxidase/100 μM hypoxanthine or to NO generated from 20 μM DEA-NO or superoxide plus NO. PGE₂ (hatched bars) and 6-keto-PGF_1α (black bars) in the media were analyzed by ELISA. Data represent means ± SEM from 12 experiments. White bars, relaxation.