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. 1999 Jul 1;190(1):1–8. doi: 10.1084/jem.190.1.1

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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AP1 transcription is prevented by S. (A) Western blots for c-jun activation/phosphorylation were performed with lysates of either nonstimulated (nst), 1 h–stimulated (IgE/Ag), or 1 h S-pretreated cells before activation (IgE/Ag + S). c-jun and phospho-c-jun are indicated by arrows (right). (B) The transcriptional activation of an AP1-dependent reporter gene (3 x TRE) was measured in transient transfections. Corrected luciferase values are indicated on the y-axis, stimuli and input DNA on the x-axis. pGL2 represents the parental vector. Cells were activated for 3 h. Data represent mean ± SD of triplicate determinations.