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. 1999 Jul 1;190(1):1–8. doi: 10.1084/jem.190.1.1

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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S1P induces the MAPK pathway and AP1 transcription factors. (A) Western blot analysis with lysates of nonstimulated (nst) or 10 μM S1P–stimulated cells for the time points indicated. MEK 1,2, phospho-MEK 1,2, erk 1,2, p-erk 1,2, jnk 1,2, p-jnk 1, c-jun, and p-c-jun are indicated by arrows (right). (B) Supershift analysis on an AP1 consensus site as radiolabeled probe with nuclear extracts of either nonstimulated (nst) or 1 h S1P–stimulated cells as indicated at the top. f, free probe. Antibodies against AP1 transcription factors are given at the top. −, activated complex without antibody.