Table 2.
Effect of IL-10 and TGF-β on IL-2 Production by Stimulated Naive Spleen Cells
| Cytokines added | Experiment 1 | Experiment 2 |
|---|---|---|
| U/ml | ||
| None | 45 | 150 |
| 30 pg/ml TGF-β1 | 30 | 97 |
| 30 pg/ml TGF-β2 | 25 | 108 |
| 1 ng/ml IL-10 | 30 | 114 |
| 1 ng/ml IL-10 + 30 pg/ml TGF-β1 | 15 | 31 |
| 1 ng/ml IL-10 + 30 pg/ml TGF-β2 | 5 | 17 |
| Day 4 supernatant | 0.1 | 0.4 |
All cultures contained 105 naive spleen cells plus 5 × 105 T-depleted spleen cells (APCs), stimulated with 1 μM PCC peptide for 48 h at 37°C. IL-2 was measured by bioassay (CTL-L). Data shown are from two out of three separate experiments.