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. 1999 Jul 1;190(1):75–90. doi: 10.1084/jem.190.1.75

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Transitional B cells of normal mice. Three-color flow cytometric analysis of cells isolated from bone marrow, blood, spleens, and lymph nodes of adult C57BL/6 mice. 100,000 events were collected. (A) Log density plots showing IgD and IgM expression. Top, all cells; center, MEL-14⁻ cells; bottom, MEL-14⁺ cells. M, mature B cells; T, transitional B cells. Arrows, cells that are indicated as T2 in panel B. (B) Cells were stained with Abs to IgM and CD21. M, mature B cells; T1, CD21⁻ and T2, CD21^bright transi-tional B cells. (C) Splenocytes were stained with Abs to IgM, IgD, and CD23. The plots show the IgD and IgM staining of cells separated on the basis of the expression of CD23. CD23⁻ B cells include IgM^bright IgD⁻ T1, and MZ B cells and all IgM⁻ cells in the spleen (T cells and macrophages). Only B cells are positive for CD23. They are mostly T2 and mature (M) B cells. (D) Splenocytes were stained with Abs to IgD, CD21, and IgM and separated into IgD⁻ or IgD⁺ cells. IgD⁻ T1 and MZ B cells can be distinguished on the basis of CD21 expression. T2 and mature B cells are both in the gate of the IgD⁺ B cells but are either bright (T2) or dull (M) for IgM.

Transitional B cells of normal mice. Three-color flow cytometric analysis of cells isolated from bone marrow, blood, spleens, and lymph nodes of adult C57BL/6 mice. 100,000 events were collected. (A) Log density plots showing IgD and IgM expression. Top, all cells; center, MEL-14⁻ cells; bottom, MEL-14⁺ cells. M, mature B cells; T, transitional B cells. Arrows, cells that are indicated as T2 in panel B. (B) Cells were stained with Abs to IgM and CD21. M, mature B cells; T1, CD21⁻ and T2, CD21^bright transi-tional B cells. (C) Splenocytes were stained with Abs to IgM, IgD, and CD23. The plots show the IgD and IgM staining of cells separated on the basis of the expression of CD23. CD23⁻ B cells include IgM^bright IgD⁻ T1, and MZ B cells and all IgM⁻ cells in the spleen (T cells and macrophages). Only B cells are positive for CD23. They are mostly T2 and mature (M) B cells. (D) Splenocytes were stained with Abs to IgD, CD21, and IgM and separated into IgD⁻ or IgD⁺ cells. IgD⁻ T1 and MZ B cells can be distinguished on the basis of CD21 expression. T2 and mature B cells are both in the gate of the IgD⁺ B cells but are either bright (T2) or dull (M) for IgM.