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. 1999 Jul 1;190(1):75–90. doi: 10.1084/jem.190.1.75

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© 1999 The Rockefeller University Press

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T1 B cells are the precursors of T2 and mature B cells. (A) Splenocytes of 1- (left) and 3- (center) wk-old and adult (right) C57BL/6 mice were stained with Abs to CD21 and IgM and analyzed by flow cytometry. (B) Splenic cells from recipient RAG-2^−/− mice were analyzed at the indicated times after transfer of 2 × 10⁶ splenic B cells from a pool of 1-wk-old mice. Cells were stained with Abs to IgM, IgD, and CD21. Top panels, IgM vs. IgD staining. Bottom panels, the CD21 vs. IgM profile of IgD⁺IgM⁺ donor B cells. 200,000 events were collected. Data is shown as dot plots to highlight the few transferred cells that home to the spleen. In the dot plots corresponding to the control (adult) spleen, only 5% of the collected events are shown.

T1 B cells are the precursors of T2 and mature B cells. (A) Splenocytes of 1- (left) and 3- (center) wk-old and adult (right) C57BL/6 mice were stained with Abs to CD21 and IgM and analyzed by flow cytometry. (B) Splenic cells from recipient RAG-2^−/− mice were analyzed at the indicated times after transfer of 2 × 10⁶ splenic B cells from a pool of 1-wk-old mice. Cells were stained with Abs to IgM, IgD, and CD21. Top panels, IgM vs. IgD staining. Bottom panels, the CD21 vs. IgM profile of IgD⁺IgM⁺ donor B cells. 200,000 events were collected. Data is shown as dot plots to highlight the few transferred cells that home to the spleen. In the dot plots corresponding to the control (adult) spleen, only 5% of the collected events are shown.