Table 1.
Cell Cycle Analysis of T1, T2, and Mature B Cells
| Mature | T1 | T2 and MZ | ||
|---|---|---|---|---|
| Control | spleen | 3 | 3 | 15 |
| bone marrow | 5 | 5 | — | |
| CD45 | spleen | 3 | 2 | 33 |
| bone marrow | 5 | 3 | — | |
| CBA/N | spleen | 4 | 3 | 21 |
| bone marrow | 7 | 5 | — | |
| Mature | T1 and MZ | T2 | ||
| Control spleen | 3 | 1 | 17 | |
| CD45spleen | 4 | 1 | 31 | |
| CBA/N spleen | 4 | 4 | 31 | |
Top: spleen and bone marrow cells were stained with Abs to IgM and CD21. After ethanol fixation, propidium iodide was used to label DNA. For cell cycle analysis, cells were gated in T1, T2, and M, as indicated in Fig. 1 B, and the DNA content was measured in these subpopulations. In bone marrow, T2 B cells are undetectable. Percentages of B cells in the G2–M phase of the cell cycle are given.
Bottom: splenic cells were stained with Abs to IgM and IgD before DNA labeling. T1, MZ, T2, and mature B cells were identified as described in the text.