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. 1999 Jul 1;190(1):101–112. doi: 10.1084/jem.190.1.101

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The enhancement effect of TCS in HEK293 cells expressing CCR5 or CXCR4. The cell membranes from CCR5 (A and C) or CXCR4 (B and D) transfected cells were stimulated with different concentrations of RANTES (A) or SDF-1α (B) in the absence or presence of 0.2 μM TCS, or incubated with different concentrations of TCS in the presence of 10 nM RANTES (C) or SDF-1α (D). The [³⁵S]GTPγS binding of each sample was then measured as described in Materials and Methods. Data were mean ± SE of three independent experiments performed in duplicate.

The enhancement effect of TCS in HEK293 cells expressing CCR5 or CXCR4. The cell membranes from CCR5 (A and C) or CXCR4 (B and D) transfected cells were stimulated with different concentrations of RANTES (A) or SDF-1α (B) in the absence or presence of 0.2 μM TCS, or incubated with different concentrations of TCS in the presence of 10 nM RANTES (C) or SDF-1α (D). The [³⁵S]GTPγS binding of each sample was then measured as described in Materials and Methods. Data were mean ± SE of three independent experiments performed in duplicate.