Figure 3.

The magnitude of f-MIGWIIA–specific CTL responses to L. monocytogenes infection is different among mouse strains. Female age-matched C57BL/6, C3H/HeJ, and BALB/c mice were infected with 2,000 L. monocytogenes, and splenocytes were obtained on the indicated day after primary infection, enriched for CD8⁺ T cells, and stained with antibodies to CD8 and CD62L as well as one of the PE-conjugated tetramers. Two mice per strain were analyzed each day. (A) The dot plots show H2-M3/f-MIGWIIA (left panels) and H2-M3/COI self (right panels) tetramer stainings for one mouse per strain on day 7 after infection. Staining for CD62L (l-selectin) is shown on the x-axis, and tetramer staining on the y-axis. Dot plots are gated on CD8⁺ lymphocytes. Percentages in the upper left quadrants represent the percentage of CD8⁺ CD62L^low (activated) cells that are tetramer positive. (B) The average number of H2-M3/f-MIGWIIA and H2-M3/COI (self) tetramer–positive cells per spleen is shown for two mice of each strain on days 5, 7, and 9 after infection. SD are indicated.