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. 1999 Jul 19;190(2):229–240. doi: 10.1084/jem.190.2.229

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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DCs from PP are more potent stimulators of allogeneic T cell proliferation than are those from SP. Mixed lymphocyte reaction was carried out with varying numbers of purified BALB/c DCs (H-2^d) and 10⁵ T cells from B10.A (H-2^k) mice per well in 96-well microtiter plates. Proliferation was measured by [³H]thymidine uptake during the last 8 h of a 48-h culture. Results are represented as the mean cpm of triplicate cultures on the y-axis, with the number of DCs per well on the x-axis. Similar results were obtained in three separate experiments conducted in the same manner.