Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Jul 19;190(2):229–240. doi: 10.1084/jem.190.2.229

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Cytokine production by OVA TCR transgenic T cells during secondary stimulation. Naive CD4⁺/MEL14⁺ FACS^®-sorted OVA TCR transgenic T cells were stimulated with purified CD11c⁺ DCs isolated from SP (A, B, and C) or PP (D, E, and F) in the presence of blocking antibodies against TGF-β, IL-10, or isotype control antibody for 5–6 d. T cells were restimulated with plate-bound anti-CD3∈ and soluble anti-CD28 antibodies for up to 48 h. Supernatants were harvested and IFN-γ (A and D), IL-4 (B and E), and IL-10 (C and F) levels were measured by ELISA at 24 (IL-4) or 48 h (IFN-γ and IL-10). The P values for cytokines secreted in the presence or absence of neutralizing antibody in the priming cultures are indicated with value labels or with an asterisk if P > 0.05. This experiment was repeated four times producing similar cytokine secretion patterns.