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. 1999 Jul 19;190(2):217–228. doi: 10.1084/jem.190.2.217

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Northern blot analysis of activated splenocytes. (A) C57BL/6 splenocytes were cultured in medium alone (medium) or supplemented with 5 μg/ml ConA (+ConA). (B) P14 splenocytes were cultured alone (−p33) or in the presence of irradiated EL4 cells coated with p33 peptide (+p33). Total RNA was extracted from cells harvested at various times during activation and from an untreated C57BL/6 thymus (Thy). The blots in A and B were hybridized with probes for ITM2A, EF1α, and CD69 (shown for A only).