Table 1.
Recognition of CD66 Receptor N-domains by MS11 Opa Variants
| Receptor type | Opa variant | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| − | A | B | C | D | E | F | G | H | I | J | K | ||
| CD66e | − | − | + | + | + | + | + | + | + | + | + | − | |
| CD66a | − | − | + | + | − | − | + | + | + | + | − | − | |
| CD66c | − | − | + | + | − | − | − | + | − | + | − | − | |
| CD66d | − | − | + | + | − | − | − | + | − | + | − | − | |
| CD66b | − | − | − | − | − | − | − | − | − | − | − | − | |
| CD66b/e | − | − | − | − | − | − | − | − | − | − | − | − | |
| CD66e/b | − | − | + | + | − | − | − | + | − | + | − | − | |
MS11 Opa variants were incubated with E. coli lysates containing the indicated His-tagged CD66 N-domain and processed for immunoblotting. Blots were probed with anti-His antibody and peroxidase-conjugated protein A followed by ECL. When a significant signal was present on the blot, recognition was defined as +. CD66a–e are native N-domain sequences; CD66b/e and CD66e/b represent chimeric N-domains between CD66b and CD66e, as described in reference 18. Some of these data were shown previously in reference 18.