Table 3.
Binding of CD66b N-domain Mutants by MS11 Opa Variants
| mut | Mutations introduced in the N-domain of CD66b | Binding by Opa variants | ||
|---|---|---|---|---|
| OpaB | OpaC | OpaI | ||
| None | − | − | − | |
| 13 | R29F+N32S | − | − | − |
| 14 | D27H+P28L+R29F+N32S | − | − | − |
| 15 | A41G+R44Q | − | − | − |
| 16 | D27H+P28L+R29F+N32S+A41G | +/− | +/− | +/− |
| 17 | D27H+P28L+R29F+N32S+R44Q | +/− | +/− | +/− |
| 18 | D27H+P28L+R29F+N32S+A41G+R44Q | ++ | ++ | ++ |
| 19 | R29F+N32S+A41G+R44Q | ++ | − | + |
| 20 | P28L+R29F+N32S+A41G+R44Q | ++ | ++ | ++ |
| 21 | D27H+R29F+N32S+A41G+R44Q | ++ | − | ++ |
MS11 Opa variants were incubated with E. coli lysates containing the indicated mutant (mut) CD66b N-domain and processed for immunoblotting. Binding was calculated by determining the signal generated by the amount of His-tagged N-domain present for each variant, compared with the signal generated in a lane where an amount of His-tagged N-domain was loaded representing 100% binding. Opa− variants did not bind any N-domain construct (data not shown). Results represent the mean of two to four independent experiments. ++, >50% binding; +, 20–50% binding; +/−, 5–20% binding; −, <5% binding.